Micropropagation as Means of Rapid Multiplication of Newly Developed Blackberry and Black Currant Cultivars
نویسندگان
چکیده
Buds from the branches cut during dormancy (end of January) were used as the initial explants and set to develop under laboratory conditions at room temperature. Aseptic culture was established on Murashige and Skoog (MS) medium with BA 2.0, IBA 0.5 and GA3 0.1 mg 1-1. MS media with BA and IBA or NAA and GA3 were used for multiplication phase, whereas medium MS with mineral salts reduced to 1/2, organic complex unchanged according to MS, with 1.0 mg 1-1 IBA, 0.1 mg 1-1 GA3 and 1g 1-1 of active charcoal was used in the rooting phase.
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